Blar i forfatter "Elvevold, Kjetil"

Viser treff 1-5 av 5

    • Autofluorescence in freshly isolated adult human liver sinusoidal cells 

      Larsen, Anett Kristin; Simon-Santamaria, Jaione; Elvevold, Kjetil; Ericzon, Bo Gøran; Mortensen, Kim Erlend; McCourt, Peter Anthony; Smedsrød, Bård; Sørensen, Karen Kristine (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-13)
      Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment ...

    • Chemical and Enzyme-Assisted Extraction of Fucoidan from two Species of Brown Macroalgae (Ascophyllum nodosum and Saccharina latissima) 

      Maribu, Ingrid; Arnesen, Jan-Arne; Elvevold, Kjetil; Haug, Tor (Conference object; Konferansebidrag, 2022)
      Macroalgae are vital species and are known to produce a wide range of bioactive compounds including polysaccharides, such as fucoidan. Traditionally, these compounds are extracted using hazardous solvents that leads to environmental pollution and waste. Considering this, more sustainable technologies are being investigated as greener alternatives.<p> <p>The main goal of this study was to ...

    • FITC conjugation markedly enhances hepatic clearance of N-formyl peptides 

      Øie, Cristina Ionica; Snapkov, Igor; Elvevold, Kjetil; Sveinbjørnsson, Baldur; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-08-05)
      In both septic and aseptic inflammation, N-formyl peptides may enter the circulation and induce a systemic inflammatory response syndrome similar to that observed during septic shock. The inflammatory response is brought about by the binding of N-formyl peptide to formyl peptide receptors (FPRs), specific signaling receptors expressed on myeloid as well as non-myeloid cells involved in the ...

    • pH-dependent structural transitions in cationic ionizable lipid mesophases are critical for lipid nanoparticle function 

      Philipp, Julian; Dabkowska, Aleksandra; Reiser, Anita; Frank, Kilian; Krzysztoń, Rafał; Brummer, Christiane; Nickel, Bert; Blanchet, Clement E.; Sudarsan, Akhil; Ibrahim, Mohd; Johansson, Svante; Skantze, Pia; Skantze, Urban; Östman, Sofia; Johansson, Marie; Henderson, Neil; Elvevold, Kjetil; Smedsrød, Bård; Schwierz, Nadine; Lindfors, Lennart; Rädler, Joachim O. (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-12-06)
      Lipid nanoparticles (LNPs) are advanced core-shell particles for messenger RNA (mRNA) based therapies that are made of polyethylene glycol (PEG) lipid, distearoylphosphatidylcholine (DSPC), cationic ionizable lipid (CIL), cholesterol (chol), and mRNA. Yet the mechanism of pH-dependent response that is believed to cause endosomal release of LNPs is not well understood. Here, we show that eGFP (enhanced ...

    • Protocol for Isolation and Culture of Mouse Hepatocytes (HCs), Kupffer Cells (KCs), and Liver Sinusoidal Endothelial Cells (LSECs) in Analyses of Hepatic Drug Distribution 

      Elvevold, Kjetil; Kyrrestad, Ingelin; Smedsrød, Bård (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-02-25)
      Development of the new generation of drugs (e.g., oligo- and polynucleotides administered intravascularly either as free compounds or as nano-formulations) frequently encounters major challenges such as lack of control of targeting and/or delivery. Uncontrolled or unwanted clearance by the liver is a well-known and particularly important hurdle in this respect. Hence, reliable techniques are needed ...